完美世界 辰东 小说,已完本玄幻小说排行榜,好看的电视剧 http://www.dadafen.cn 生化試劑、耗材專業(yè)供應商 Mon, 30 Oct 2023 11:59:53 +0000 zh-CN hourly 1 https://wordpress.org/?v=6.2.6 伯樂Bio-rad Aminex色譜柱 http://www.dadafen.cn/16762.html Mon, 30 Oct 2023 11:59:53 +0000 http://www.dadafen.cn/?p=16762 繼續(xù)閱讀 ]]> 伯樂Bio-rad Aminex色譜柱

上海金畔生物代理美國Bio-Rad 伯樂產品,歡迎新老客戶訪問美國Bio-Rad 伯樂官網或者咨詢我們獲取更多相關產品信息。

Aminex糖分析柱
由聚苯乙烯二乙烯苯樹脂填裝而成的Aminex HPLC柱,具有良好的高壓及pH穩(wěn)定性、高柱效和選擇性。Aminex HPLC柱通過離子調節(jié)分配層析技術分離混合物,這種技術是根據多種不同的化學特性分離分子。離子調節(jié)分配層析技術的分離機制包括離子排阻、離子交換、正相和反相分配、分子量排阻和配體交換。根據層析的物理和化學特性, 可選擇使用一個或多個分離機制。Aminex柱是工業(yè)界普遍使用的,用來分析碳水化合物、有機酸、有機堿和其它包括肽和核酸的有機小分子的標準產品。用Aminex柱分離復雜混合物需要使用簡單等流相(常用水),并要求精確控溫。
研究過程中,樣品可重復的保留值和分辨率是至關重要的。不同批次間及不同柱間的測試證明Aminex 柱是值得信賴的產品。
Aminex柱的使用壽命長,并能保持上百次試驗的可重復性。耐用的層析柱避免了頻繁更換柱引起的數據變化和高成本。
Aminex柱應用實例
? 木漿水解產物分析
? 玉米汁成分分離
? 大鼠肝流出液層析圖譜
? 從cheddar 奶酪中分離有機酸

糖分析色譜柱
125-0143 Aminex HPX-87N Column, 300×7.8mm 蔗糖 9 鈉 8 5–9
125-0142 Aminex HPX-87K Column, 300×7.8mm 糖蜜、玉米糖漿 9 鉀 8 5–9
125-0095 Aminex HPX-87C Column, 300×7.8mm 高濃度果糖玉米糖漿 9 鈣 8 5–9
125-0094 Aminex HPX-87C Column, 250×4.0mm 糖醇 (USP收錄) 9 鈣 8 5–9
125-0098 Aminex HPX-87P Column, 300×7.8mm 戊糖(類), 纖維素水解產物 9 鉛 8 5–9
125-0140 Aminex HPX-87H Column, 300×7.8mm 含有機酸的糖 9 氫 8 1–3
125-0096 Aminex HPX-42C Column, 300×7.8mm 低聚糖,增稠劑 25 鈣 4 5–9
125-0097 Aminex HPX-42A Column, 300×7.8mm 低聚糖 到D-11 25 銀 4 6–8
125-0105 Fast Carbohydrate Column, 100×7.8mm 葡萄糖、半乳糖、蔗糖、果糖 9 鉛 8 5–9

Aminex糖、有機酸和醇分析色譜柱

貨號 產品描述 分析對象
125-0140 Aminex HPX-87H Column, 300 x 7.8 mm 含有機酸的糖、發(fā)酵過程監(jiān)測
125-0143 Aminex HPX-87N Column, 300 x 7.8 mm 蔗糖
125-0142 Aminex HPX-87K Column, 300 x 7.8 mm 糖蜜、玉米糖漿
125-0095 Aminex HPX-87C Column, 300 x 7.8 mm 高濃度果糖玉米糖漿
125-0094 Aminex HPX-87C Column, 250 x 4.0 mm 糖醇(USP收錄)
125-0098 Aminex HPX-87P Column, 300 x 7.8 mm 戊糖(類), 纖維素水解產物
125-0096 Aminex HPX-42C Column, 300 x 7.8 mm 低聚糖,增稠劑
125-0097 Aminex HPX-42A Column, 300 x 7.8 mm 低聚糖到D-11
125-0105 Fast Carbohydrate Column, 100 x 7.8 mm 葡萄糖、半乳糖、蔗糖、果糖
125-0100 Fast Acid Analysis Column , 100 x 7.8 mm 乙醇、乙二醇、有機酸
125-0115 Fermentation Monitoring Column , 150 x 7.8 mm 糖、酸、醇類

 

Aminex有機酸和醇分析柱
125–0140 Aminex HPX–87H Column, 300×7.8mm 含有有機酸的糖、發(fā)酵過程監(jiān)測 9 氫 8 1–3
125–0100 Fast Acid Analysis Column, 100×7.8mm 乙醇、乙二醇、有機酸分析 9 氫 8 1–3
125–0115 Fermentation Monitoring Column, 150×7.8mm 糖、酸、醇類 9 氫 8 1–3
*包括一個層析柱和2 個Micro-Guard 濾柱;需要標準濾柱支架,貨號#125-0131。
** 需要De-ashing 濾柱支架,貨號#125-0139。
***IG =工業(yè)純;顆粒更大。

 

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美國Bio-Rad 伯樂 1620137 PFGE Certified Agarose, 100g http://www.dadafen.cn/16756.html Mon, 30 Oct 2023 05:25:56 +0000 http://www.dadafen.cn/?p=16756 繼續(xù)閱讀 ]]> 美國Bio-Rad 伯樂 1620137 PFGE Certified Agarose, 100g

上海金畔生物代理美國Bio-Rad 伯樂產品,歡迎新老客戶訪問美國Bio-Rad 伯樂官網或者咨詢我們獲取更多相關產品信息。

脈沖場電泳瓊脂糖。Agarose for separation of large DNA in pulsed field gel applications, certified free of inhibitors, DNases, Rnases, 100 g

Code / SKU Description 2107RMB報價
Biorad bio-rad 伯樂 1351303 PUREBLU DAPI NUCLEAR STAIN.DYE ¥1,570.00
Biorad bio-rad 伯樂 1351304 PUREBLU HOECHST33342 NUCLR DYE ¥1,498.00
Biorad bio-rad 伯樂 1450003 Cell Ctng Kit, Ctng Slides & Trypan Blue ¥673.00
Biorad bio-rad 伯樂 1450005 THERMAL PRINTER, 100-240V, CC ¥9,275.00
Biorad bio-rad 伯樂 1450014 VERIFICATION SLIDE,CELL COUNT. ¥7,374.00
Biorad bio-rad 伯樂 1450015 Cell Ctng Slides, 5 x 30 2-well slides ¥3,176.00
Biorad bio-rad 伯樂 1450016 Cell Ctng Slides, 10 x 30 2-well slides ¥5,912.00
Biorad bio-rad 伯樂 1450017 Cell Ctng Slides, 20 x 30 2-well slides ¥11,455.00
Biorad bio-rad 伯樂 1450018 Cell Ctng Slides, 30 x 30 2-well slides ¥16,326.00
Biorad bio-rad 伯樂 1450019 Cell Ctng Slides, 40 x 30 2-well slides ¥21,762.00
Biorad bio-rad 伯樂 1450020 Cell Ctng Slides, 80 x 30 2-well slides ¥42,653.00
Biorad bio-rad 伯樂 1450021 Trypan Blue 5 x 1.5 ml ¥485.00
Biorad bio-rad 伯樂 1450022 Trypan Blue 10 x 1.5 ml ¥826.00
Biorad bio-rad 伯樂 1703350 TRANSFECTIN REAGENT,0.5ML ¥2,333.00
Biorad bio-rad 伯樂 1703351 TRANSFECTIN REAGENT,1.0ML ¥3,786.00
Biorad bio-rad 伯樂 1703352 TRANSFECTIN REAGENT,5X1.0ML ¥15,608.00
Biorad bio-rad 伯樂 1703360 siLentFect LIPID REAGENT,0.5ML ¥2,862.00
Biorad bio-rad 伯樂 1703361 siLentFect LIPID REAGENT,1.0ML ¥4,154.00
Biorad bio-rad 伯樂 1703362 siLentFect LIPID RGNT,5X 1.0ML ¥18,470.00
Biorad bio-rad 伯樂 1652081 Gene Pulser/MicroPulser Cuvettes ¥225.00
Biorad bio-rad 伯樂 1652082 Gene Pulser/MicroPulser Cuvettes ¥225.00
Biorad bio-rad 伯樂 1652083 Gene Pulser/MicroPulser Cuvettes ¥225.00
Biorad bio-rad 伯樂 1652086 CUVETTES 2MM PKG 50 STERILE ¥2,207.00
Biorad bio-rad 伯樂 1652088 CUVETTES 4MM PKG 50 STERILE ¥2,207.00
Biorad bio-rad 伯樂 1652089 CUVETTES 1MM PKG 50 STERILE ¥2,207.00
Biorad bio-rad 伯樂 1652091 Gene Pulser Cuvettes 0.4 cm, pkg 500 ¥16,765.00
Biorad bio-rad 伯樂 1652092 Gene Pulser Cuvettes 0.2 cm, pkg 500 ¥16,765.00
Biorad bio-rad 伯樂 1652093 Gene Pulser Cuvettes 0.1 cm, pkg 500 ¥16,765.00
Biorad bio-rad 伯樂 1652225 HEPTA ADAPTOR,BIOLISTIC ¥156,975.00
Biorad bio-rad 伯樂 1652226 STOP SCREEN,HEPTA,BIOLISTIC ¥8,226.00
Biorad bio-rad 伯樂 1652244 Helios Control Bullets 1.6um Gold pkg 12 ¥2,709.00
Biorad bio-rad 伯樂 1652262 0.6 μm Gold Microcarriers 0.25 g ¥5,786.00
Biorad bio-rad 伯樂 1652263 1.0 μm Gold Microcarriers 0.25 g ¥5,786.00
Biorad bio-rad 伯樂 1652264 1.6 μm Gold Microcarriers 0.25 g ¥5,786.00
Biorad bio-rad 伯樂 1652266 Tungsten M-10 Microcarriers ~0.7 μm, 6 g ¥1,050.00
Biorad bio-rad 伯樂 1652267 Tungsten M-17 Microcarriers ~1.1 μm, 6 g ¥1,050.00
Biorad bio-rad 伯樂 1652268 Tungsten M-20 Microcarriers ~1.3 μm, 6 g ¥1,050.00
Biorad bio-rad 伯樂 1652269 Tungsten M-25 Microcarriers ~1.7 μm, 6 g ¥960.00
Biorad bio-rad 伯樂 1652278 Optimization Kit for 500 Bombardments ¥23,044.00
Biorad bio-rad 伯樂 1652322 Biolistic Micro Carrier Holder, pkg 5 ¥2,324.00
Biorad bio-rad 伯樂 1652323 Biolistic PDS-1000/He Disk-Vac System ¥2,844.00
Biorad bio-rad 伯樂 1652326 450 PSI Rupture Disks Qty 100 ¥1,534.00
Biorad bio-rad 伯樂 1652327 650 PSI Rupture Disks Qty 100 ¥1,534.00
Biorad bio-rad 伯樂 1652328 900 PSI Rupture Disks Qty 100 ¥1,534.00
Biorad bio-rad 伯樂 1652329 1100 PSI Rupture Disks Qty 100 ¥1,534.00
Biorad bio-rad 伯樂 1652330 1350 PSI Rupture Disks Qty 100 ¥1,534.00
Biorad bio-rad 伯樂 1652331 1550 PSI Rupture Disks Qty 100 ¥1,534.00
Biorad bio-rad 伯樂 1652332 1800 PSI Rupture Disks Qty 100 ¥1,534.00
Biorad bio-rad 伯樂 1652333 2000 PSI Rupture Disks Qty 100 ¥1,534.00
Biorad bio-rad 伯樂 1652334 2200 PSI Rupture Disks Qty 100 ¥1,534.00
Biorad bio-rad 伯樂 1652335 PDS-1000/He Macrocarriers, pkg 500 ¥6,387.00
Biorad bio-rad 伯樂 1652336 PDS-1000/He Stopping Screens, pkg 500 ¥3,965.00
Biorad bio-rad 伯樂 1652417 Helios Gene Gun Barrel Liner, pkg 5 ¥1,534.00
Biorad bio-rad 伯樂 1652421 Syringe Kit, 5 ¥906.00
Biorad bio-rad 伯樂 1652422 Helios Tubing Cutter ¥2,252.00
Biorad bio-rad 伯樂 1652424 Helios Gene Gun Optimization Kit ¥19,394.00
Biorad bio-rad 伯樂 1652440 Helios Gene Gun Cartrridge Kit ¥3,391.00
Biorad bio-rad 伯樂 1652441 Tefzel Tubing, 15 m ¥2,952.00
Biorad bio-rad 伯樂 1652475 Helios Gene Gun Diffusion Screens ¥1,185.00
Biorad bio-rad 伯樂 1652676 Gene Pulser Electroporation Buf 10×1.8ml ¥3,445.00
Biorad bio-rad 伯樂 1652677 Gene Pulser Electroporation Buffer 30 ml ¥5,024.00
Biorad bio-rad 伯樂 1652681 GP Mxcell 96-Well Electroporation Plate ¥4,790.00
Biorad bio-rad 伯樂 1652682 Gene Pulser MXcell 24-Well Plate ¥4,252.00
Biorad bio-rad 伯樂 1652683 12-Well Electroporation Plate ¥4,620.00
Biorad bio-rad 伯樂 1702480 DNA Quantitation Kit ¥1,552.00
Biorad bio-rad 伯樂 1613100 Certified Molecular Biology Agarose ¥270.00
Biorad bio-rad 伯樂 1613101 Certified Molecular Biology Agarose ¥1,346.00
Biorad bio-rad 伯樂 1613102 Certified Molecular Biology Agarose ¥5,382.00
Biorad bio-rad 伯樂 1613103 Certified PCR Agarose ¥996.00
Biorad bio-rad 伯樂 1613104 Certified PCR Agarose ¥3,920.00
Biorad bio-rad 伯樂 1613105 Certified PCR Agarose ¥14,397.00
Biorad bio-rad 伯樂 1613106 Certified Low Range Ultra Agarose ¥1,059.00
Biorad bio-rad 伯樂 1613107 Certified Low Range Ultra Agarose ¥4,189.00
Biorad bio-rad 伯樂 1613108 Certified Megabase Agarose ¥485.00
Biorad bio-rad 伯樂 1613109 Certified Megabase Agarose ¥1,911.00
Biorad bio-rad 伯樂 1613110 Certified Megabase Agarose ¥6,351.00
Biorad bio-rad 伯樂 1613111 Certified Low-Melt Agarose ¥1,525.00
Biorad bio-rad 伯樂 1613112 Certified Low-Melt Agarose ¥6,728.00
Biorad bio-rad 伯樂 1613113 Certified PCR Low-Melt Agarose ¥1,382.00
Biorad bio-rad 伯樂 1613114 Certified PCR Low-Melt Agarose ¥3,974.00
Biorad bio-rad 伯樂 1613115 Certified PCR Low-Melt Agarose ¥15,402.00
Biorad bio-rad 伯樂 1620022 Zero MR Agarose for IEP/IEF, 10 g ¥2,144.00
Biorad bio-rad 伯樂 1620100 Standard Low MR Agarose IEP/IEF, 100g ¥1,292.00
Biorad bio-rad 伯樂 1620102 Standard Low MR Agarose IEP/IEF, 500g ¥5,427.00
Biorad bio-rad 伯樂 1620137 PFGE Certified Agarose, 100g ¥1,678.00
Biorad bio-rad 伯樂 1620138 Pulsed Field Certified Agarose ¥7,679.00
Biorad bio-rad 伯樂 1703591 CHEF Mammal Genomic DNA Plug Kt for PFGE ¥3,346.00
Biorad bio-rad 伯樂 1703592 CHEF Bac Genomic DNA Plug Kit for PFGE ¥4,279.00
Biorad bio-rad 伯樂 1703593 CHEF Yeast Genomic DNA Plug Kit for PFGE ¥5,589.00
Biorad bio-rad 伯樂 1703594 Cleancut Agarose 2%, 12 ml ¥781.00
Biorad bio-rad 伯樂 1703597 Bacterial Genomic DNA Module for PFGE ¥1,355.00
Biorad bio-rad 伯樂 1703598 Yeast Genomic DNA Module for PFGE ¥2,243.00
Biorad bio-rad 伯樂 1814030 OPTICALLY CLEAR HEAT SEAL ¥1,651.00
Biorad bio-rad 伯樂 1814035 PERMANENT CLEAR HEAT SEAL ¥1,651.00
Biorad bio-rad 伯樂 1814040 Pierceable Foil Heat Seal ¥1,202.00
Biorad bio-rad 伯樂 1814080 Sealing Frame, PX1 PCR Plate Sealer ¥915.00
Biorad bio-rad 伯樂 1814085 PX1 PCR Plate Sealer Support Block ¥2,979.00
Biorad bio-rad 伯樂 2239441 PCR PLT,96WELL,25/CASE,iCyr iQ ¥1,140.00
Biorad bio-rad 伯樂 2239442 96-Well PCR Plate Sealing Mats, pkg 5 ¥557.00
Biorad bio-rad 伯樂 2239444 TAPE,OPTICAL SEALING,iCycler ¥906.00
Biorad bio-rad 伯樂 12001925 Droplet DigitalTM PCR Plates 96-Well, Semi-Skirted ¥1,947.00
Biorad bio-rad 伯樂 ADR3296 OPTICAL COMPRESSION PAD,96-WEL ¥207.00
Biorad bio-rad 伯樂 ADR3841 MICROSEAL 384 PLATE POSITIONER ¥1,409.00
Biorad bio-rad 伯樂 ADR5001 PRESSURE PAD,FOR MSA-5001,1/8″ ¥243.00
Biorad bio-rad 伯樂 CHO1401 CHILL-OUT LIQUID WAX,RED,100ML ¥341.00
Biorad bio-rad 伯樂 CHO1404 CHILL-OUT LIQUID WAX,RED,1-LTR ¥2,270.00
Biorad bio-rad 伯樂 CHO1411 CHILL-OUT LIQUID WAX,CLR,100ML ¥637.00
Biorad bio-rad 伯樂 CHO1414 CHILL-OUT LIQUID WAX,CLR,1-LTR ¥2,019.00
Biorad bio-rad 伯樂 CON9601 CONCORD MICROPLATE,96-WELL ¥2,144.00
Biorad bio-rad 伯樂 ECT1000 CAP TOOL,APPLICATION & REMOVAL ¥207.00
Biorad bio-rad 伯樂 ECT2000 Strip Cap Tool ¥557.00
Biorad bio-rad 伯樂 HSL9601 HS 96 FAST,CLR/CLR,no BARCODE ¥763.00
Biorad bio-rad 伯樂 HSL9605 HS 96 FAST,CLR/WHT,no BARCODE ¥790.00
Biorad bio-rad 伯樂 HSL9641 HS 96-Well, Semi-Skirt LP Green shell, Clear well ¥763.00
Biorad bio-rad 伯樂 HSL9645 HS 96 FAST,GRN/WHT,no BARCODE ¥790.00
Biorad bio-rad 伯樂 HSL9901 HS 96-Well, Semi-Skirt LP Clear shell, Clear well, barcoded ¥862.00
Biorad bio-rad 伯樂 HSL9905 HS 96 FAST,CLR/WHT,wBARCODE ¥897.00
Biorad bio-rad 伯樂 HSP3801 HSP-384,CLR/CLR 50/BX ¥1,481.00
Biorad bio-rad 伯樂 HSP3801B Hard-Shell 384W Skrtd Plts Bulk Clr/Clr ¥13,760.00
Biorad bio-rad 伯樂 HSP3805 Hard-Shell Thin-Wall 384-Well Skirted PCR Plates ¥2,126.00
Biorad bio-rad 伯樂 HSP3811 HSP-384,RED/CLR 50/BX ¥1,570.00
Biorad bio-rad 伯樂 HSP3821 HSP-384,YEL/CLR 50/BX ¥1,570.00
Biorad bio-rad 伯樂 HSP3831 HSP-384,BLU/CLR 50/BX ¥1,570.00
Biorad bio-rad 伯樂 HSP3841 HSP-384,GRN/CLR 50/BX ¥1,570.00
Biorad bio-rad 伯樂 HSP3865 Hard-Shell Thin-Wall 384-Well Skirted PCR Plates ¥1,624.00
Biorad bio-rad 伯樂 HSP3866 Hard-Shell Thin-Wall 384-Well Skirted PCR Plates ¥1,570.00
Biorad bio-rad 伯樂 HSP3901 HSP-384,CLR/CLR,BARCODE,50/BX ¥1,570.00
Biorad bio-rad 伯樂 HSP3905 HSP-384,CLR/WHT,BARCODE,50/BX ¥2,046.00
Biorad bio-rad 伯樂 HSP9601 Hard-Shell Thin-Wall 96-Well Skirted PCR Plates ¥1,669.00
Biorad bio-rad 伯樂 HSP9601B Hard-Shell 96W Low Skrtd Wht/Clr/Bk ¥13,563.00
Biorad bio-rad 伯樂 HSP9611 Hard-Shell 96W Low Skrtd Plts Red/Clr ¥1,669.00
Biorad bio-rad 伯樂 HSP9621 Hard-Shell 96W Low Skrtd Plts Yel/Clr ¥1,669.00
Biorad bio-rad 伯樂 HSP9631 Hard-Shell 96W Low Skrtd Plts Blu/Clr ¥1,669.00
Biorad bio-rad 伯樂 HSP9635 Hard-Shell Thin-Wall 96-Well Skirted PCR Plates ¥1,669.00
Biorad bio-rad 伯樂 HSP9641 Hard-Shell 96W Low Skrtd Plts Grn/Clr ¥1,669.00
Biorad bio-rad 伯樂 HSP9645 Hard-Shell 96W Low Skrtd Plts Grn/Wht ¥1,669.00
Biorad bio-rad 伯樂 HSP9655 Hard-Shell 96W Low Skrtd Wht/Wht ¥1,669.00
Biorad bio-rad 伯樂 HSP9661 Hard-Shell 96W Low Skrtd Plts Blk/Clr ¥1,669.00
Biorad bio-rad 伯樂 HSP9665 Hard-Shell 96W Low Skrtd Plts Blk/Wht ¥1,669.00
Biorad bio-rad 伯樂 HSP9666 HSP-96,BLK/BLK 50/BX ¥1,669.00
Biorad bio-rad 伯樂 HSP9901 Hard-Shell 96W Low Skrtd Wht/Clr/BC ¥1,777.00
Biorad bio-rad 伯樂 HSP9901B Hard-Shell 96W Low Skrtd Wht/Clr/BC/Bk ¥14,182.00
Biorad bio-rad 伯樂 HSP9911B HSP-96,BARCODE RED/CLR 400/BX ¥16,649.00
Biorad bio-rad 伯樂 HSP9931B HSP-96,BARCODE BLU/CLR 400/BX ¥16,649.00
Biorad bio-rad 伯樂 HSP9955 Hard-Shell Thin-Wall 96-Well Skirted PCR Plates ¥1,777.00
Biorad bio-rad 伯樂 HSR4801 HSR-480,CLR/CLR,BARCODE,50/BX ¥1,481.00
Biorad bio-rad 伯樂 HSR4801K Hard-Shell 384-Well 480 PCR Plates Kit ¥4,638.00
Biorad bio-rad 伯樂 HSR4805 HSR-480,CLR/WHT,BARCODE,50/BX ¥2,126.00
Biorad bio-rad 伯樂 HSR4805K Hard-Shell 384-Well 480 PCR Plates Kit ¥4,638.00
Biorad bio-rad 伯樂 HSR9901 Hard Shell 96-Well 480 PCR Plates, clear shell, clear well ¥1,032.00
Biorad bio-rad 伯樂 HSR9901K Hard-Shell 96W 480 Plts Crl/Clr BC ¥4,943.00
Biorad bio-rad 伯樂 HSR9905 HSR 96 480,CLR/WHT,w/ BARCODE ¥1,059.00
Biorad bio-rad 伯樂 HSR9905K Hard-Shell 96W 480 Plts Crl/Wht BC ¥5,006.00
Biorad bio-rad 伯樂 HSS9601 Hard-Shell 96W Hi SmSkrtd Plts Clr/Clr ¥808.00
Biorad bio-rad 伯樂 HSS9641 Hard-Shell 96W Hi SmSkrtd Plts Grn/Clr ¥799.00
Biorad bio-rad 伯樂 HSS9665 Hard-Shell 96W Hi SmSkrtd Plts Blk/Wht ¥1,032.00
Biorad bio-rad 伯樂 HSS9901 Hard-Shell 96W Hi SmSkrtd Crl/Clr BC ¥853.00
Biorad bio-rad 伯樂 MLL4801 Multiplate Low-Profile 48-Well Unskirted PCR Plates ¥1,409.00
Biorad bio-rad 伯樂 MLL4851 Multiplate 48W Low UnSkrtd Plts White ¥1,534.00
Biorad bio-rad 伯樂 MLL9601 Multiplate Low-Profile 96-Well Unskirted PCR Plates ¥978.00
Biorad bio-rad 伯樂 MLL9651 LOW MULTIPLATE-96,WHITE 25/BX ¥1,122.00
Biorad bio-rad 伯樂 MLP4801 Multiplate 48W Hi UnSkrtd Plts Clear ¥1,651.00
Biorad bio-rad 伯樂 MLP9601 MULTIPLATE-96,NATL 25/BX ¥978.00
Biorad bio-rad 伯樂 MLP9611 MULTIPLATE-96,PINK 25/BX ¥1,032.00
Biorad bio-rad 伯樂 MLP9621 MULTIPLATE-96,YELLOW 25/BX ¥1,032.00
Biorad bio-rad 伯樂 MLP9631 Multiplate 96-Well Unskirted PCR Plates ¥1,032.00
Biorad bio-rad 伯樂 MLP9641 MULTIPLATE-96,GREEN 25/BX ¥1,032.00
Biorad bio-rad 伯樂 MLP9651 Multiplate 96-Well Unskirted PCR Plates ¥1,274.00
Biorad bio-rad 伯樂 MSA5001 MICROSEAL A SEALING FILM,50/PK ¥960.00
Biorad bio-rad 伯樂 MSB1001 Microseal ‘B’ Adhesive Seals ¥1,176.00
Biorad bio-rad 伯樂 MSC1001 MICROSEAL C OPTICAL FILM ¥1,651.00
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Biorad bio-rad 伯樂 MSS9601 Microseal 96-Well PCR Plates ¥1,167.00
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Biorad bio-rad 伯樂 SLF0201 FRAME-SEAL,9X9MM 25UL ¥987.00
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Biorad bio-rad 伯樂 SLF1201 FRAME-SEAL,17X28MM 125UL ¥1,328.00
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Biorad bio-rad 伯樂 TBC0802 0.2 ml 8-Tube Strips and Domed Cap Strips ¥2,055.00
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Biorad bio-rad 伯樂 TBI0501 0.5 ml Tubes With Flat Caps ¥575.00
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Biorad bio-rad 伯樂 TBS0201 0.2 ml 8-Tube Strips Without Caps ¥718.00
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Biorad bio-rad 伯樂 TBS0221 TUBE,STRIP-8,YEL 120 STRIPS ¥718.00
Biorad bio-rad 伯樂 TBS0231 0.2 ml 8-Tube Strips Without Caps ¥718.00
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Biorad bio-rad 伯樂 TCS0801 CAP STRIP-8,DOMED 130 STRIPS ¥458.00
Biorad bio-rad 伯樂 TCS0803 Optical Flat 8-Cap Strips, pkg 120 ¥350.00
Biorad bio-rad 伯樂 TCS1201 CAP,STRIP-12,DOMED,200 STRIPS ¥826.00
Biorad bio-rad 伯樂 TFI0201 TUBE,.2ML FLAT-CAP NAT 1000/PK ¥521.00
Biorad bio-rad 伯樂 TLS0801 Low-Profile 0.2 ml 8-Tube Strips Without Caps ¥817.00
Biorad bio-rad 伯樂 TLS0851 TUBE,STRIP-8,LO-PRO WHT 120/PK ¥897.00
Biorad bio-rad 伯樂 TRC0501 RACK W/COVER,96-PLACE,5/PK ¥521.00
Biorad bio-rad 伯樂 TWI0201 TUBE,.2ML,DOME-CAP NAT 1000/PK ¥521.00
Biorad bio-rad 伯樂 1708870 iTaq DNA Polymerase ¥1,427.00
Biorad bio-rad 伯樂 1708872 MgCl<SUB>2</SUB> Solution ¥476.00
Biorad bio-rad 伯樂 1708874 dNTP Mix ¥736.00
Biorad bio-rad 伯樂 1708875 iTaq DNA Polymerase ¥23,556.00
Biorad bio-rad 伯樂 1725300 iProof High Fidelity DNA Polymerase ¥225.00
Biorad bio-rad 伯樂 1725301 iProof High-Fidelity DNA Polymerase ¥1,014.00
Biorad bio-rad 伯樂 1725302 iProof High-Fidelity DNA Polymerase ¥4,593.00
Biorad bio-rad 伯樂 1725330 iProof High-Fidelity PCR Kit ¥1,077.00
Biorad bio-rad 伯樂 1725331 iProof High-Fidelity PCR Kit ¥3,364.00
Biorad bio-rad 伯樂 1725393 5x iProof HPLC HF Buffer ¥279.00
Biorad bio-rad 伯樂 1725394 5x iProof HPLC GC Buffer ¥279.00
Biorad bio-rad 伯樂 1849001 Tube Frame 96 Deep W Reaction Module ¥1,194.00
Biorad bio-rad 伯樂 1845080 CFX Automation System II Installation Qualification/Operational Qualification (IQ/OQ) Kit ¥13,330.00
Biorad bio-rad 伯樂 1845081 CFX Automation System II Operational Qualification (OQ) Kit ¥12,541.00
Biorad bio-rad 伯樂 1845082 CFX Automation System II Installation Qualification/Operational Qualification (IQ/OQ) Service and Kit ¥13,043.00
Biorad bio-rad 伯樂 1845083 CFX Automation System II Operational Qualification (OQ) Service and Kit ¥12,541.00
Biorad bio-rad 伯樂 1845384 CFX384 Optical Rxn Module w Consumables ¥196,408.00
Biorad bio-rad 伯樂 1862000 T100 Tube Support Ring ¥1,391.00
Biorad bio-rad 伯樂 1845098 CFX QUAL PLATE 96 W KIT ¥8,970.00
Biorad bio-rad 伯樂 1845099 CFX QUAL PLATE 384 W KIT ¥9,536.00
Biorad bio-rad 伯樂 1653644 OUTERGLASSPLATE38X30CM ¥1,507.00
Biorad bio-rad 伯樂 1653645 OUTERPLATE21X40CM ¥1,490.00
Biorad bio-rad 伯樂 1653646 OUTER PLATE 21 X 50 CM ¥1,615.00
Biorad bio-rad 伯樂 1653688 N-COMB,14CM,36 WELL,0.4MM THK ¥969.00
Biorad bio-rad 伯樂 1653698 W-COMB,31CM,80 WELL,0.4MM THK ¥1,086.00
Biorad bio-rad 伯樂 1653702 SHARKTOOTH COMB 48 WELL X .4MM ¥924.00
Biorad bio-rad 伯樂 1653812 SPACERS,PVC,30CM,0.4MM,RED ¥655.00
Biorad bio-rad 伯樂 1653836 SHARKTOOTH COMB,PVC,24W,.40MM ¥700.00
Biorad bio-rad 伯樂 1653837 SHARKTOOTH COMB,PVC,36W,.40MM ¥700.00
Biorad bio-rad 伯樂 1653838 SHARKTOOTH COMB,PVC,48W,.40MM ¥826.00
Biorad bio-rad 伯樂 1653841 SHRKTH CMB,PVC,30CM,97W,.40MM ¥1,238.00
Biorad bio-rad 伯樂 1653845 S/T COMB,MP,34 WELL,0.4MM,RED ¥826.00
Biorad bio-rad 伯樂 1653875 CLAMP SET,30CM,SEQUI-GEN GT ¥4,979.00
Biorad bio-rad 伯樂 1653877 SEQUI-GEN SE CLAMP SET, 50CM ¥5,382.00
Biorad bio-rad 伯樂 1703623 CHEF Prep Comb, 2 Wells for Standards ¥906.00
Biorad bio-rad 伯樂 1703627 CHEF 15-well Comb, 21 cm W x 1.5 mm ¥1,086.00
Biorad bio-rad 伯樂 1703628 CHEF 30-well Comb, 21 cm W x 1.5 mm ¥1,086.00
Biorad bio-rad 伯樂 1703643 Gel Scoop ¥1,579.00
]]>
Vector Lab代理Aleuria Aurantia Lectin (AAL), Agarose bound http://www.dadafen.cn/29527.html Wed, 16 Feb 2022 12:23:26 +0000 http://www.dadafen.cn/29527.html 繼續(xù)閱讀 ]]> Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統(tǒng)引領了生物學檢測方法的重大革命,該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng),并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Aleuria Aurantia Lectin (AAL), Agarose bound

AL-1393-2

SKU Unit Size Price Qty
AL-1393-2 2 ml

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Aleuria Aurantia Lectin (AAL), Agarose bound

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

Features:

  • Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2×107 daltons
  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation. ?This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • 2 mg lectin/ml gel
  • Inhibiting/Eluting Sugar: 100 mM L-fucose?or?Glycoprotein Eluting Solution (ES-3100)

?

Specifications

More Information
Unit Size 2 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.08% sodium azide
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-3100. Alternatively, 100 mM L-fucose in buffered saline can be used. After use, wash the gel with several column volumes of buffered saline then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity Fucose, Arabinose
Conjugate Agarose

Documents

  • Safety Data Sheet
  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Download CoA
  • Datasheet

Product FAQs

I purchased an agarose bound lectin from Vector Labs. Do you have a protocol outline on how this may be applied in a column format?

Our agarose lectin products are supplied as hydrated matrix solutions in amber glass bottles. The agarose (bead) material will settle and you will see two phases in the tube supplied. The upper phase is buffer. A column can be prepared in a commercial plastic device such as Bio-Rad Cat # 732-6008 or an inverted Pasteur pipet with glass wool lightly packed in the neck to retain the agarose. 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity.(Sometimes an air bubble in the column tip prevents flow; tapping the column should get the flow started). 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. 3) Place a collection vessel (e.g. glass test tube) under the column tip and apply the glycoprotein-containing solution.Allow the solution to drain through using gravity. We recommend against pushing or pulling the material through the column. Retain the flow through material until the desired binding has been confirmed. 4) After sample application, wash column with 2-3 column volumes of buffer (or until the absorbance at 280nm is reduced to a satisfactory level) to remove unbound materials before elution. 5) Place a fresh collection vessel under the column tip.Apply the eluting solution again letting gravity do the work of moving the solution over the column. Note that in some cases, several column volumes of eluting solution may be required to achieved adequate release of bound material. 6) Following elution, the column can be prepared for reuse by washing with 10 column volumes of buffer. 7) If the column is to be stored, equilibrate the column with buffer containing 0.08% sodium azide. Cover the column with a plastic wrap, or similar, to prevent desiccation and keep at 4 degrees Celsius. The column will be stable for many months when stored under these conditions.

What are recommended conditions for using the agarose-lectin in chromatography?

The pH should be near neutral, the maximum pressure for packing the resin is 10 psi, and the maximum flow rate 3.5 ml/min.

Related Products

Aleuria Aurantia Lectin (AAL), Agarose bound

L-fucose Catalog #: S-9007

Aleuria Aurantia Lectin (AAL), Agarose bound

Aleuria Aurantia Lectin (AAL), Biotinylated Catalog #: B-1395-1

Aleuria Aurantia Lectin (AAL), Agarose bound

Aleuria Aurantia Lectin (AAL), Unconjugated Catalog #: L-1390-2

Aleuria Aurantia Lectin (AAL), Agarose bound

Aleuria Aurantia Lectin (AAL), Fluorescein Catalog #: FL-1391-1

Citations

Aleuria Aurantia Lectin (AAL), Agarose bound Powered by Bioz See more details on Bioz

Technical Information

This lectin is a dimer of two identical subunits of about 36 kDa each. Unlike Ulex europaeus and Lotus tetragonolobus lectins which prefer (α -1,2) linked fucose residues, Aleuria aurantia lectin binds preferentially to fucose linked (α -1,6) to N-acetylglucosamine or to fucose linked (α -1,3) to N-acetyllactosamine related structures. AAL also reversibly binds fucose attached to nucleic acids

Agarose bound* Aleuria Aurantia lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

Inhibiting/Eluting Sugar: 100 mM L-fucose

*2 mg lectin/ml gel

Home All Products Aleuria aurantia (AAL) Aleuria Aurantia Lectin (AAL), Agarose bound
]]>
Vector Lab代理Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound http://www.dadafen.cn/29519.html Wed, 16 Feb 2022 11:28:20 +0000 http://www.dadafen.cn/29519.html 繼續(xù)閱讀 ]]> Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統(tǒng)引領了生物學檢測方法的重大革命,該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng),并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound

AL-1143-2

SKU Unit Size Price Qty
AL-1143-2 2 ml

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound

Description
Specifications
Documents
Product FAQs
Related Products
Technical Information

Description

Agarose bound Erythrina cristagalli lectin?is prepared using 4% agarose beads.?Erythrina cristagalli lectin consists of two different subunits of approximately 28 kDa and 26 kDa. The carbohydrate structure to which ECL binds is frequently found in membrane and serum glycoproteins of mammalian origin.

Features:

  • Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2×107 daltons
  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation. ?This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • Inhibiting/Eluting Sugar: 200 mM lactose?or?Glycoprotein Eluting Solution (ES-1100)

?

Specifications

More Information
Unit Size 2 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM lactose, 0.08% sodium azide
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 0.2 M lactose can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity Galactose, N-Acetylgalactosamine, Lactose
Conjugate Agarose

Documents

  • Safety Data Sheet
  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Download CoA
  • Datasheet

Product FAQs

I purchased an agarose bound lectin from Vector Labs. Do you have a protocol outline on how this may be applied in a column format?

Our agarose lectin products are supplied as hydrated matrix solutions in amber glass bottles. The agarose (bead) material will settle and you will see two phases in the tube supplied. The upper phase is buffer. A column can be prepared in a commercial plastic device such as Bio-Rad Cat # 732-6008 or an inverted Pasteur pipet with glass wool lightly packed in the neck to retain the agarose. 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity.(Sometimes an air bubble in the column tip prevents flow; tapping the column should get the flow started). 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. 3) Place a collection vessel (e.g. glass test tube) under the column tip and apply the glycoprotein-containing solution.Allow the solution to drain through using gravity. We recommend against pushing or pulling the material through the column. Retain the flow through material until the desired binding has been confirmed. 4) After sample application, wash column with 2-3 column volumes of buffer (or until the absorbance at 280nm is reduced to a satisfactory level) to remove unbound materials before elution. 5) Place a fresh collection vessel under the column tip.Apply the eluting solution again letting gravity do the work of moving the solution over the column. Note that in some cases, several column volumes of eluting solution may be required to achieved adequate release of bound material. 6) Following elution, the column can be prepared for reuse by washing with 10 column volumes of buffer. 7) If the column is to be stored, equilibrate the column with buffer containing 0.08% sodium azide. Cover the column with a plastic wrap, or similar, to prevent desiccation and keep at 4 degrees Celsius. The column will be stable for many months when stored under these conditions.

What are recommended conditions for using the agarose-lectin in chromatography?

The pH should be near neutral, the maximum pressure for packing the resin is 10 psi, and the maximum flow rate 3.5 ml/min.

Related Products

Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound

Galactose Catalog #: S-9003

Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound

Lactose Catalog #: S-9004

Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound

Erythrina Cristagalli Lectin (ECL, ECA), Biotinylated Catalog #: B-1145-5

Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound

Erythrina Cristagalli Lectin (ECL, ECA), Fluorescein Catalog #: FL-1141-5

Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound

Erythrina Cristagalli Lectin (ECL, ECA), Unconjugated Catalog #: L-1140-10

Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound

Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100

Technical Information

Sialic acid substitution on this structure appears to prevent the lectin from binding. This specificity offers an opportunity to utilize agarose bound ECL to isolate or fractionate mammalian glycoproteins.

This lectin has been reported to be useful for the isolation of human natural killer (NK) cells using a negative selection panning technique (protocol available upon request or on our website). Human NK cells appear to lack accessible surface carbohydrate structures required for binding ECL and, unlike other mononuclear cells, do not adhere to ECL-coated culture dishes. Since this procedure involves a negative selection panning technique, a high recovery of viable NK cells can be obtained. The adherent cells can also be recovered by incubation in galactose or lactose.

Agarose bound* Erythrina cristagalli lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

*3 mg lectin/ml gel

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

?

Home All Products Erythrina cristagalli lectin (ECL) Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound
]]>
Vector Lab代理Soybean Agglutinin (SBA), Agarose bound http://www.dadafen.cn/29513.html Wed, 16 Feb 2022 10:17:56 +0000 http://www.dadafen.cn/29513.html 繼續(xù)閱讀 ]]> Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統(tǒng)引領了生物學檢測方法的重大革命,該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng),并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Soybean Agglutinin (SBA), Agarose bound

AL-1013-2

SKU Unit Size Price Qty
AL-1013-2 2 ml

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Soybean Agglutinin (SBA), Agarose bound

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

Agarose bound Soybean agglutinin is prepared using our affinity-purified lectins.?Composed of four subunits of approximately equal size, soybean agglutinin is a family of closely related isolectins. This glycoprotein has a molecular weight of about 120 kDa and an isoelectric point near pH 6.0. SBA preferentially binds to oligosaccharide structures with terminal α- or β-linked N-acetylgalactosamine, and to a lesser extent, galactose residues. Binding can be blocked by substitutions on penultimate sugars, such as fucose attached to the penultimate galactose in blood group B substance.

Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation. ?This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • Inhibiting/Eluting Sugar:?200 mM?N-acetylgalactosamine or Glycoprotein Eluting Solution (ES-2100)

?

Specifications

More Information
Unit Size 2 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM galactose, 0.08% sodium azide, 0.01 mM MnCl2
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 0.2 M N-acetylgalactoseamine in buffered saline can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity Galactose, N-Acetylgalactosamine
Conjugate Agarose

Documents

  • Safety Data Sheet
  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Download CoA
  • Datasheet

Product FAQs

I purchased an agarose bound lectin from Vector Labs. Do you have a protocol outline on how this may be applied in a column format?

Our agarose lectin products are supplied as hydrated matrix solutions in amber glass bottles. The agarose (bead) material will settle and you will see two phases in the tube supplied. The upper phase is buffer. A column can be prepared in a commercial plastic device such as Bio-Rad Cat # 732-6008 or an inverted Pasteur pipet with glass wool lightly packed in the neck to retain the agarose. 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity.(Sometimes an air bubble in the column tip prevents flow; tapping the column should get the flow started). 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. 3) Place a collection vessel (e.g. glass test tube) under the column tip and apply the glycoprotein-containing solution.Allow the solution to drain through using gravity. We recommend against pushing or pulling the material through the column. Retain the flow through material until the desired binding has been confirmed. 4) After sample application, wash column with 2-3 column volumes of buffer (or until the absorbance at 280nm is reduced to a satisfactory level) to remove unbound materials before elution. 5) Place a fresh collection vessel under the column tip.Apply the eluting solution again letting gravity do the work of moving the solution over the column. Note that in some cases, several column volumes of eluting solution may be required to achieved adequate release of bound material. 6) Following elution, the column can be prepared for reuse by washing with 10 column volumes of buffer. 7) If the column is to be stored, equilibrate the column with buffer containing 0.08% sodium azide. Cover the column with a plastic wrap, or similar, to prevent desiccation and keep at 4 degrees Celsius. The column will be stable for many months when stored under these conditions.

What are recommended conditions for using the agarose-lectin in chromatography?

The pH should be near neutral, the maximum pressure for packing the resin is 10 psi, and the maximum flow rate 3.5 ml/min.

Related Products

Soybean Agglutinin (SBA), Agarose bound

N-acetylgalactosamine Catalog #: S-9001

Soybean Agglutinin (SBA), Agarose bound

Soybean Agglutinin (SBA), Biotinylated Catalog #: B-1015-5

Soybean Agglutinin (SBA), Agarose bound

Soybean Agglutinin (SBA), Fluorescein Catalog #: FL-1011-2

Soybean Agglutinin (SBA), Agarose bound

Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100

Citations

Soybean Agglutinin (SBA), Agarose bound Powered by Bioz See more details on Bioz

Technical Information

An important application for SBA is the separation of pluripotent stem cells from human bone marrow. Cells fractionated by SBA do not produce graft vs host disease and can be used in bone marrow transplantation across histocompatibility barriers.

Agarose bound* Soybean agglutinin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

*4 mg lectin/ml gel

?

Home All Products Soybean agglutinin (SBA) Soybean Agglutinin (SBA), Agarose bound
]]>
Vector Lab代理Wisteria Floribunda Lectin (WFA, WFL), Agarose bound http://www.dadafen.cn/29506.html Wed, 16 Feb 2022 09:23:46 +0000 http://www.dadafen.cn/29506.html 繼續(xù)閱讀 ]]> Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統(tǒng)引領了生物學檢測方法的重大革命,該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng),并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Wisteria Floribunda Lectin (WFA, WFL), Agarose bound

AL-1353-2

SKU Unit Size Price Qty
AL-1353-2 2 ml

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Wisteria Floribunda Lectin (WFA, WFL), Agarose bound

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

Features:

  • Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2×107 daltons
  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation. ?This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • 3 mg lectin/ml gel
  • Inhibiting/Eluting Sugar: 200 mM?N-acetylgalactosamine?or?Glycoprotein Eluting Solution (ES-2100)

?

Specifications

More Information
Unit Size 2 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM galactose, 0.08% sodium azide
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution, Cat. No. ES-2100. Alternatively, 200 mM N-acetylgalactoseamine or several column volumes of 200 mM acetic acid can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity N-Acetylgalactosamine
Conjugate Agarose

Documents

  • Safety Data Sheet
  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Download CoA
  • Datasheet

Product FAQs

I purchased an agarose bound lectin from Vector Labs. Do you have a protocol outline on how this may be applied in a column format?

Our agarose lectin products are supplied as hydrated matrix solutions in amber glass bottles. The agarose (bead) material will settle and you will see two phases in the tube supplied. The upper phase is buffer. A column can be prepared in a commercial plastic device such as Bio-Rad Cat # 732-6008 or an inverted Pasteur pipet with glass wool lightly packed in the neck to retain the agarose. 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity.(Sometimes an air bubble in the column tip prevents flow; tapping the column should get the flow started). 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. 3) Place a collection vessel (e.g. glass test tube) under the column tip and apply the glycoprotein-containing solution.Allow the solution to drain through using gravity. We recommend against pushing or pulling the material through the column. Retain the flow through material until the desired binding has been confirmed. 4) After sample application, wash column with 2-3 column volumes of buffer (or until the absorbance at 280nm is reduced to a satisfactory level) to remove unbound materials before elution. 5) Place a fresh collection vessel under the column tip.Apply the eluting solution again letting gravity do the work of moving the solution over the column. Note that in some cases, several column volumes of eluting solution may be required to achieved adequate release of bound material. 6) Following elution, the column can be prepared for reuse by washing with 10 column volumes of buffer. 7) If the column is to be stored, equilibrate the column with buffer containing 0.08% sodium azide. Cover the column with a plastic wrap, or similar, to prevent desiccation and keep at 4 degrees Celsius. The column will be stable for many months when stored under these conditions.

What are recommended conditions for using the agarose-lectin in chromatography?

The pH should be near neutral, the maximum pressure for packing the resin is 10 psi, and the maximum flow rate 3.5 ml/min.

Related Products

Wisteria Floribunda Lectin (WFA, WFL), Agarose bound

N-acetylgalactosamine Catalog #: S-9001

Wisteria Floribunda Lectin (WFA, WFL), Agarose bound

Wisteria Floribunda Lectin (WFA, WFL), Unconjugated Catalog #: L-1350-5

Wisteria Floribunda Lectin (WFA, WFL), Agarose bound

Wisteria Floribunda Lectin (WFA, WFL), Fluorescein Catalog #: FL-1351-2

Wisteria Floribunda Lectin (WFA, WFL), Agarose bound

Wisteria Floribunda Lectin (WFA, WFL), Biotinylated Catalog #: B-1355-2

Wisteria Floribunda Lectin (WFA, WFL), Agarose bound

Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100

Citations

Wisteria Floribunda Lectin (WFA, WFL), Agarose bound Powered by Bioz See more details on Bioz

Technical Information

The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.

Agarose bound* WFL is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

*3 mg lectin/ml gel

Home All Products Wisteria floribunda (WFA) Wisteria Floribunda Lectin (WFA, WFL), Agarose bound
]]>
Vector Lab代理Ulex Europaeus Agglutinin I (UEA I), Agarose bound http://www.dadafen.cn/28906.html Mon, 14 Feb 2022 00:07:36 +0000 http://www.dadafen.cn/28906.html 繼續(xù)閱讀 ]]> Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統(tǒng)引領了生物學檢測方法的重大革命,該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng),并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Ulex Europaeus Agglutinin I (UEA I), Agarose bound

AL-1063-2

SKU Unit Size Price Qty
AL-1063-2 2 ml

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Ulex Europaeus Agglutinin I (UEA I), Agarose bound

Description
Specifications
Documents
Product FAQs
Related Products
Technical Information

Description

Agarose bound?Ulex europaeus agglutinin is prepared using our affinity-purified lectins.?Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.

Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Product supplied as a 1:1 suspension in buffer
  • Inhibiting/Eluting Sugar: 50 mM – 100 mM L-fucose?or?Glycoprotein Eluting Solution (ES-3100)

?

Specifications

More Information
Unit Size 2 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.1 mM CaCl2, 0.08% sodium azide
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-3100. Alternatively, 100 mM L-fucose in buffered saline can be used. After use, wash the gel with several column volumes of buffered saline then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity Fucose, Arabinose
Conjugate Agarose

Documents

  • Safety Data Sheet
  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Download CoA
  • Datasheet

Product FAQs

I purchased an agarose bound lectin from Vector Labs. Do you have a protocol outline on how this may be applied in a column format?

Our agarose lectin products are supplied as hydrated matrix solutions in amber glass bottles. The agarose (bead) material will settle and you will see two phases in the tube supplied. The upper phase is buffer. A column can be prepared in a commercial plastic device such as Bio-Rad Cat # 732-6008 or an inverted Pasteur pipet with glass wool lightly packed in the neck to retain the agarose. 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity.(Sometimes an air bubble in the column tip prevents flow; tapping the column should get the flow started). 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. 3) Place a collection vessel (e.g. glass test tube) under the column tip and apply the glycoprotein-containing solution.Allow the solution to drain through using gravity. We recommend against pushing or pulling the material through the column. Retain the flow through material until the desired binding has been confirmed. 4) After sample application, wash column with 2-3 column volumes of buffer (or until the absorbance at 280nm is reduced to a satisfactory level) to remove unbound materials before elution. 5) Place a fresh collection vessel under the column tip.Apply the eluting solution again letting gravity do the work of moving the solution over the column. Note that in some cases, several column volumes of eluting solution may be required to achieved adequate release of bound material. 6) Following elution, the column can be prepared for reuse by washing with 10 column volumes of buffer. 7) If the column is to be stored, equilibrate the column with buffer containing 0.08% sodium azide. Cover the column with a plastic wrap, or similar, to prevent desiccation and keep at 4 degrees Celsius. The column will be stable for many months when stored under these conditions.

What are recommended conditions for using the agarose-lectin in chromatography?

The pH should be near neutral, the maximum pressure for packing the resin is 10 psi, and the maximum flow rate 3.5 ml/min.

Related Products

Ulex Europaeus Agglutinin I (UEA I), Agarose bound

L-fucose Catalog #: S-9007

Ulex Europaeus Agglutinin I (UEA I), Agarose bound

Ulex Europaeus Agglutinin I (UEA I), Biotinylated Catalog #: B-1065-2

Ulex Europaeus Agglutinin I (UEA I), Agarose bound

Ulex Europaeus Agglutinin I (UEA I), Rhodamine Catalog #: RL-1062-2

Ulex Europaeus Agglutinin I (UEA I), Agarose bound

Ulex Europaeus Agglutinin I (UEA I), DyLight? 594 Catalog #: DL-1067-1

Ulex Europaeus Agglutinin I (UEA I), Agarose bound

Ulex Europaeus Agglutinin I (UEA I), DyLight? 649 Catalog #: DL-1068-1

Technical Information

Agarose bound* Ulex europaeus agglutinin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

*2 mg lectin/ml gel

Home All Products Ulex europaeus I (UEA I) Ulex Europaeus Agglutinin I (UEA I), Agarose bound
]]>
Vector Lab代理Vicia Villosa Lectin (VVL, VVA), Agarose bound http://www.dadafen.cn/28442.html Fri, 11 Feb 2022 20:52:29 +0000 http://www.dadafen.cn/28442.html 繼續(xù)閱讀 ]]> Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統(tǒng)引領了生物學檢測方法的重大革命,該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng),并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Vicia Villosa Lectin (VVL, VVA), Agarose bound

AL-1233-2

SKU Unit Size Price Qty
AL-1233-2 2 ml

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Vicia Villosa Lectin (VVL, VVA), Agarose bound

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

Agarose bound?Vicia villosa lectin is prepared using our affinity-purified lectins. This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen).?

Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation. ?This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • Inhibiting/Eluting Sugar: 200 mM?N-acetylgalactosamine?or?Glycoprotein Eluting Solution (ES-2100)

?

Specifications

More Information
Unit Size 2 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM galactose, 0.08% sodium azide
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution, Cat. No. ES-2100. Alternatively, 200 mM N-acetylgalactosamine can be used.Elution can also be achieved with 100 mM sodium acetate, pH 3.0, 1 M galactose. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity N-Acetylgalactosamine
Conjugate Agarose

Documents

  • Safety Data Sheet
  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Download CoA
  • Datasheet

Product FAQs

I purchased an agarose bound lectin from Vector Labs. Do you have a protocol outline on how this may be applied in a column format?

Our agarose lectin products are supplied as hydrated matrix solutions in amber glass bottles. The agarose (bead) material will settle and you will see two phases in the tube supplied. The upper phase is buffer. A column can be prepared in a commercial plastic device such as Bio-Rad Cat # 732-6008 or an inverted Pasteur pipet with glass wool lightly packed in the neck to retain the agarose. 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity.(Sometimes an air bubble in the column tip prevents flow; tapping the column should get the flow started). 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. 3) Place a collection vessel (e.g. glass test tube) under the column tip and apply the glycoprotein-containing solution.Allow the solution to drain through using gravity. We recommend against pushing or pulling the material through the column. Retain the flow through material until the desired binding has been confirmed. 4) After sample application, wash column with 2-3 column volumes of buffer (or until the absorbance at 280nm is reduced to a satisfactory level) to remove unbound materials before elution. 5) Place a fresh collection vessel under the column tip.Apply the eluting solution again letting gravity do the work of moving the solution over the column. Note that in some cases, several column volumes of eluting solution may be required to achieved adequate release of bound material. 6) Following elution, the column can be prepared for reuse by washing with 10 column volumes of buffer. 7) If the column is to be stored, equilibrate the column with buffer containing 0.08% sodium azide. Cover the column with a plastic wrap, or similar, to prevent desiccation and keep at 4 degrees Celsius. The column will be stable for many months when stored under these conditions.

What are recommended conditions for using the agarose-lectin in chromatography?

The pH should be near neutral, the maximum pressure for packing the resin is 10 psi, and the maximum flow rate 3.5 ml/min.

Related Products

Vicia Villosa Lectin (VVL, VVA), Agarose bound

N-acetylgalactosamine Catalog #: S-9001

Vicia Villosa Lectin (VVL, VVA), Agarose bound

Vicia Villosa Lectin (VVL, VVA), Unconjugated Catalog #: L-1230-5

Vicia Villosa Lectin (VVL, VVA), Agarose bound

Vicia Villosa Lectin (VVL, VVA), Fluorescein Catalog #: FL-1231-2

Vicia Villosa Lectin (VVL, VVA), Agarose bound

Vicia Villosa Lectin (VVL, VVA), Biotinylated Catalog #: B-1235-2

Vicia Villosa Lectin (VVL, VVA), Agarose bound

Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100

Citations

Vicia Villosa Lectin (VVL, VVA), Agarose bound Powered by Bioz See more details on Bioz

Technical Information

Agarose bound* Vicia villosa lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

*3 mg lectin/ml gel

Home All Products Vicia villosa (VVL, VVA) Vicia Villosa Lectin (VVL, VVA), Agarose bound
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Vector Lab代理Sambucus Nigra Lectin (SNA, EBL), Agarose bound http://www.dadafen.cn/28231.html Thu, 10 Feb 2022 18:14:51 +0000 http://www.dadafen.cn/28231.html 繼續(xù)閱讀 ]]> Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統(tǒng)引領了生物學檢測方法的重大革命,該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng),并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Sambucus Nigra Lectin (SNA, EBL), Agarose bound

AL-1303-2

SKU Unit Size Price Qty
AL-1303-2 2 ml

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Sambucus Nigra Lectin (SNA, EBL), Agarose bound

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

Agarose bound?Sambucus nigra lectin is prepared using our affinity-purified lectins.?Sambucus nigra lectin, isolated from elderberry bark,?binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin appears to bind sialic acid linked to?N-acetylgalactosamine or galactose.?

Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation. ?This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • 3 mg lectin/ml gel
  • 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid or?Glycoprotein Eluting Solution (ES-7100)

?

Specifications

More Information
Unit Size 2 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.08% sodium azide, 20 mM lactose
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-7100. Alternatively, 0.5 M lactose in buffered saline followed by 0.5 M lactose in 0.2 M acetic acid can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline, containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity Sialic Acid
Conjugate Agarose

Documents

  • Safety Data Sheet
  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Download CoA
  • Datasheet

Product FAQs

I purchased an agarose bound lectin from Vector Labs. Do you have a protocol outline on how this may be applied in a column format?

Our agarose lectin products are supplied as hydrated matrix solutions in amber glass bottles. The agarose (bead) material will settle and you will see two phases in the tube supplied. The upper phase is buffer. A column can be prepared in a commercial plastic device such as Bio-Rad Cat # 732-6008 or an inverted Pasteur pipet with glass wool lightly packed in the neck to retain the agarose. 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity.(Sometimes an air bubble in the column tip prevents flow; tapping the column should get the flow started). 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. 3) Place a collection vessel (e.g. glass test tube) under the column tip and apply the glycoprotein-containing solution.Allow the solution to drain through using gravity. We recommend against pushing or pulling the material through the column. Retain the flow through material until the desired binding has been confirmed. 4) After sample application, wash column with 2-3 column volumes of buffer (or until the absorbance at 280nm is reduced to a satisfactory level) to remove unbound materials before elution. 5) Place a fresh collection vessel under the column tip.Apply the eluting solution again letting gravity do the work of moving the solution over the column. Note that in some cases, several column volumes of eluting solution may be required to achieved adequate release of bound material. 6) Following elution, the column can be prepared for reuse by washing with 10 column volumes of buffer. 7) If the column is to be stored, equilibrate the column with buffer containing 0.08% sodium azide. Cover the column with a plastic wrap, or similar, to prevent desiccation and keep at 4 degrees Celsius. The column will be stable for many months when stored under these conditions.

What are recommended conditions for using the agarose-lectin in chromatography?

The pH should be near neutral, the maximum pressure for packing the resin is 10 psi, and the maximum flow rate 3.5 ml/min.

Related Products

Sambucus Nigra Lectin (SNA, EBL), Agarose bound

Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins Catalog #: ES-2100-100

Sambucus Nigra Lectin (SNA, EBL), Agarose bound

Galactose Catalog #: S-9003

Sambucus Nigra Lectin (SNA, EBL), Agarose bound

Lactose Catalog #: S-9004

Sambucus Nigra Lectin (SNA, EBL), Agarose bound

Sambucus Nigra Lectin (SNA, EBL), Unconjugated Catalog #: L-1300-5

Sambucus Nigra Lectin (SNA, EBL), Agarose bound

Sambucus Nigra Lectin (SNA, EBL), Fluorescein Catalog #: FL-1301-2

Sambucus Nigra Lectin (SNA, EBL), Agarose bound

Sambucus Nigra Lectin (SNA, EBL), Biotinylated Catalog #: B-1305-2

Sambucus Nigra Lectin (SNA, EBL), Agarose bound

Sambucus Nigra Lectin (SNA, EBL), CY?3 Catalog #: CL-1303-1

Sambucus Nigra Lectin (SNA, EBL), Agarose bound

Sambucus Nigra Lectin (SNA, EBL), CY?5 Catalog #: CL-1305-1

Sambucus Nigra Lectin (SNA, EBL), Agarose bound

Glycoprotein Eluting Solution for Sialic Acid Binding Lectins Catalog #: ES-7100-100

Citations

Sambucus Nigra Lectin (SNA, EBL), Agarose bound Powered by Bioz See more details on Bioz

Technical Information

Our?coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

Elution: 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid

?

Home All Products Sambucus nigra agglutinin (SNA) Sambucus Nigra Lectin (SNA, EBL), Agarose bound
]]>
Vector Lab代理Galanthus Nivalis Lectin (GNL), Agarose bound http://www.dadafen.cn/28034.html Wed, 09 Feb 2022 17:34:47 +0000 http://www.dadafen.cn/28034.html 繼續(xù)閱讀 ]]> Vector Labs代理,歡迎訪問Vector Labs官網或者咨詢我們獲取相關Vector Labs產品信息以及報價。
Vector Labs 公司是世界著名的生物學檢測試劑公司,七十年代其在全球首推的生物素-親和素系統(tǒng)引領了生物學檢測方法的重大革命,該系統(tǒng)被普遍視為目前最靈敏、最可靠與最有效的染色系統(tǒng),并被廣泛應用于免疫組織化學、免疫電鏡、原位雜交與凝集素化學中。該系統(tǒng)仍在不斷地發(fā)展,以滿足廣大研究人員的各種不同要求。Vector 公司還是世界上凝集素產品的主要供應商,并提供大量的新產品,如酶底物、神經元示蹤劑及蛋白質、糖類與核酸的標記、分離與檢測試劑。

Galanthus Nivalis Lectin (GNL), Agarose bound

AL-1243-5

SKU Unit Size Price Qty
AL-1243-5 5 ml

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Galanthus Nivalis Lectin (GNL), Agarose bound

Description
Specifications
Documents
Product FAQs
Related Products
Citations
Technical Information

Description

Agarose bound?Galanthus nivalis lectin is prepared using our affinity-purified lectins.?Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca++ or Mn++ for binding. Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues.?

In contrast to most mannose-binding lectins, GNL will not bind α-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is α2-macroglobulin. GNL binds to many viral glycoproteins.

Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation. ?This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • 3 mg lectin/ml gel
  • Inhibiting/Eluting Sugar: 100 mM – 200 mM α-methylmannoside?or?Glycoprotein Eluting Solution (ES-1100)

?

?

?

Specifications

More Information
Unit Size 5 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 20 mM mannose, 0.08% sodium azide
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-1100. Alternatively, 0.1 M α methyl mannoside can be used.For those glycoconjugates having a very high affinity for GNL, it may be necessary to lower the pH of the eluting sugar solution to pH 4.0 with acetic acid and increase the concentration of the α methyl mannoside to 0.5 M. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity Mannose
Conjugate Agarose

Documents

  • Safety Data Sheet
  • Lectins in Histochemistry, ELISA, and Western Blot Applications
  • Download CoA
  • Datasheet

Product FAQs

I purchased an agarose bound lectin from Vector Labs. Do you have a protocol outline on how this may be applied in a column format?

Our agarose lectin products are supplied as hydrated matrix solutions in amber glass bottles. The agarose (bead) material will settle and you will see two phases in the tube supplied. The upper phase is buffer. A column can be prepared in a commercial plastic device such as Bio-Rad Cat # 732-6008 or an inverted Pasteur pipet with glass wool lightly packed in the neck to retain the agarose. 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity.(Sometimes an air bubble in the column tip prevents flow; tapping the column should get the flow started). 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. 3) Place a collection vessel (e.g. glass test tube) under the column tip and apply the glycoprotein-containing solution.Allow the solution to drain through using gravity. We recommend against pushing or pulling the material through the column. Retain the flow through material until the desired binding has been confirmed. 4) After sample application, wash column with 2-3 column volumes of buffer (or until the absorbance at 280nm is reduced to a satisfactory level) to remove unbound materials before elution. 5) Place a fresh collection vessel under the column tip.Apply the eluting solution again letting gravity do the work of moving the solution over the column. Note that in some cases, several column volumes of eluting solution may be required to achieved adequate release of bound material. 6) Following elution, the column can be prepared for reuse by washing with 10 column volumes of buffer. 7) If the column is to be stored, equilibrate the column with buffer containing 0.08% sodium azide. Cover the column with a plastic wrap, or similar, to prevent desiccation and keep at 4 degrees Celsius. The column will be stable for many months when stored under these conditions.

What are recommended conditions for using the agarose-lectin in chromatography?

The pH should be near neutral, the maximum pressure for packing the resin is 10 psi, and the maximum flow rate 3.5 ml/min.

Related Products

Galanthus Nivalis Lectin (GNL), Agarose bound

Galanthus Nivalis Lectin (GNL), Unconjugated Catalog #: L-1240-5

Galanthus Nivalis Lectin (GNL), Agarose bound

α-methylmannoside Catalog #: S-9005

Galanthus Nivalis Lectin (GNL), Agarose bound

Galanthus Nivalis Lectin (GNL), Fluorescein Catalog #: FL-1241-2

Galanthus Nivalis Lectin (GNL), Agarose bound

Glycoprotein Eluting Solution for Mannose/Glucose Binding Lectins Catalog #: ES-1100-100

Galanthus Nivalis Lectin (GNL), Agarose bound

Galanthus Nivalis Lectin (GNL), Biotinylated Catalog #: B-1245-2

Citations

Galanthus Nivalis Lectin (GNL), Agarose bound Powered by Bioz See more details on Bioz

Technical Information

Our?coupling method provides several advantages over the traditional cyanogen bromide procedure:

  • Maximum carbohydrate binding activity of the coupled lectins is retained
  • Linkage is stable over a range of pH values
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

Inhibiting/Eluting Sugar: 100 mM - 200 mM α-methylmannoside

?

Home Galanthus nivalis lectin (GNL) Galanthus Nivalis Lectin (GNL), Agarose bound
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