Lumiprobe 磺酸基-Cy5-N-羥基琥珀酰亞胺酯 Sulfo-Cyanine5 NHS ester
水溶Cyanine 5琥珀酰亞胺酯（succinimidyl ester（SE）），類似于Cy5? NHS ester，不需要有機(jī)溶劑即在水相中即可對(duì)各種帶氨基分子進(jìn)行標(biāo)記。因此該產(chǎn)品尤其適用于對(duì)那些在有機(jī)溶劑中易變性的蛋白和低溶解度蛋白的標(biāo)記。Sulfo-Cyanine 5是Cy5?的衍生物，與很多儀器如讀板機(jī)（plate readers），顯微鏡和成像設(shè)備等相兼容。是最受歡迎的熒光染料之一。
該染料是高度親水的、水溶的。非磺化的衍生物：Cyanine 5 NHS ester?？梢栽谒械膽?yīng)用中替代Cy5?，Alexa Fluor 647和DyLight 649。
Lumiprobe 磺酸基-Cy5-N-羥基琥珀酰亞胺酯 Sulfo-Cyanine5 NHS ester ? 一般特性
| 外觀: | dark blue powder |
| 分子量: | 761.84 |
| 分子式: | C36H40N3NaO10S2 |
| 溶解性: | very good in water, good in DMF and DMSO |
| 質(zhì)量控制: | NMR 1H (95%) and 13C, TLC, functional testing |
| 儲(chǔ)存條件: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
光譜特性
| 最大激發(fā)波長, nm: | 646 |
| 在最大激發(fā)波長下的消光系數(shù), Lmol-1cm-1: | 271000 |
| 最大發(fā)生波長, nm: | 662 |
| 熒光量子產(chǎn)率: | 0.2 |
| CF260: | 0.13 |
| CF280: | 0.13 |
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