Cy7-N-羥基琥珀酰胺酯|CAS 477908-53-5|Sulfo-cyanine7 NHS ester
產(chǎn)品簡介
胺類活性染料，近紅外熒光染料，Cy7?的改進的類似物。近紅外熒光素可以被用來利用生物組織的近紅外窗口。在這個光譜區(qū)域，高度增強的組織透明度使得活體成像成為可能。該染料可用于制備CY7標(biāo)記的生物分子來進行后續(xù)試驗，如活體研究及藥物開發(fā)相關(guān)實驗。Cy7的特殊結(jié)構(gòu)加固了中心聚亞甲基（polymethyne）鏈的穩(wěn)定性。這種增強的分子結(jié)構(gòu)會使其量子產(chǎn)率較其母結(jié)構(gòu)提供20%，因此它的熒光強度會更大。
該試劑在標(biāo)記時需要共溶劑有機溶劑。水溶Cy7 NHS ester可以用于蛋白NIR標(biāo)記。
Amine reactive Cyanine7, near infrared fluorescent dye, an improved analog of Cy7?.
NIR fluorophores can be used to take advantage of near infrared window of biological tissues – increased transparency of tissues in this spectral region allows to carry out in vivo imaging.
This reagent can be utilized to produce Cyanine7-labeled biomolecules for subsequent use in various in vivo research, and drug design related experiments.
The structure of Cyanine7 features rigidized design of central polymethyne chain. This molecular reinforcement allows to increase quantum yield by 20% compared with parent structure, increasing fluorescence brightness.
This reagent requires organic co-solvent for the labeling (please see Recommended Protocols section below). Water-soluble Cyanine7 NHS ester is also available, and recommended for protein NIR labeling.
一般特性
| ?外觀: | green powder |
| 分子量: | 682.29 |
| 分子式: | C41H48ClN3O4 |
| 溶解性: | soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility ? in water |
| 質(zhì)量控制: | NMR 1H (95%) and 13C, TLC, functional testing |
| 儲存條件: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for ? up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
光譜特性
| 激發(fā)波長, nm: | 750 |
| 在激發(fā)波長下的消光系數(shù), Lmol-1cm-1: | 199000 |
| 發(fā)生波長, nm: | 773 |
| 熒光量子產(chǎn)率: | 0.3 |